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DTSTAMP:20260623T230005Z
UID:f924a95c-aac7-4b3c-8ccf-b8a81b5a9f22
DTSTART:20191009T090000Z
DTEND:20191011T170000Z
DESCRIPTION:Educators: \nSebastian Jünemann (Bielefeld University)\, Dr. A
 lex Sczyrba (Bielefeld University)\, Sebastian Jaenicke (Giessen Universit
 y)\, Nils Kleinboelting (Bielefeld University) \n\nLocation: \nBielefeld U
 niversity\, Universitätsstraße 25\, 33615 Bielefeld\, Room V6-113\n\nDat
 e: \nOctober the 9th to 11th\, 2019\n\nContent:\nThe aim of this 3-day wor
 kshop will be to give students a brief overview of the tools and bioinform
 atics techniques available for the analysis of next generation sequence (N
 GS) data from microbial communities. The format will comprise a mixture of
  lectures and hands-on tutorials where students will process example data 
 sets in real-time in the de.NBI cloud environment. After covering general 
 aspects of sequence based analysis (e.g. pre-processing\, quality measurem
 ents\, error handling\, and so on) the course is divided into two parts: t
 argeted (16S rRNA gene amplicons) and untargeted (whole-genome shotgun\; W
 GS) metagenome analysis. On demand\, a compact introduction into the Linux
  operating system and the usage of the command line interface will be give
 n upstream to the introducing part to guarantee a consistent baseline for 
 the following lectures. The main aspects of the 16S part are the common pi
 peline steps beginning with pre-processing and filtering followed by OTU c
 lustering\, taxonomic classification\, and different statistical measureme
 nts. Then\, the new ASV/zOTU approach will be introduced followed by simil
 ar statistics and both approaches discussed with the attendants in conclus
 ion. In the third part\, advantages and disadvantages of whole metagenome 
 sequencing will be illustrated. As WGS metagenomics has the potential to a
 ddress the full spectra of genome-based issues\, the focus here will be on
  taxonomic and functional analysis with the aid of different bioinformatic
  tools. Two different techniques to analyze WGS metagenome data are part o
 f this section: (1) in the read-based approach the software solution MGX\,
  an integrated platform for metagenome analysis and data visualization\, w
 ill be demonstrated. (2) the assembly-based approach to potentially recove
 r near-complete genome by assembling reads into contigs which are subject 
 to binning methods to group individual contigs into genome bins. \n\nPrere
 quisites:\n- basic knowledge in microbiology and NGS-based analysis\n- pra
 ctical experience in a Linux/Unix derivatives\, the command line interface
  and file system required\n\nPrerequisites for the workshop plus the optio
 nal Linux introduction:\n- basic knowledge in microbiology and NGS-based a
 nalysis\n\nPlease not that participation to this course does not depend on
  the actual Linux experience of the applicant. Both\, experienced as well 
 as inexperienced Linux users are encouraged to submit an application. Howe
 ver\, the Linux introducing session in the morning of the first day is opt
 ional only for applicants fulfilling the Linux prerequisite.\n\nApplicatio
 n:\nPlease send your application to denbi-courses@cebitec.uni-bielefeld.de
  (subject MG­course2019) including a short motivation\, a few words about
  your background\, your level of experience on the command line (Linux/Uni
 x)\, and your experience within the field of metagenomic analysis and rela
 ted bioinformatic tools. There will be no participation fee\, yet travel a
 nd accommodation expenses need to be paid by the participants.
LOCATION:Bielefeld
SUMMARY:4th de.NBI Training Course on Metagenome Analysis
URL;VALUE=URI:https://www.denbi.de/training/649-4th-de-nbi-training-course-
 on-metagenome-analysis
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