BEGIN:VCALENDAR VERSION:2.0 PRODID:icalendar-ruby CALSCALE:GREGORIAN BEGIN:VEVENT DTSTAMP:20260808T184347Z UID:900b0e65-314b-4d74-bf0f-f9eb96cded7e DTSTART:20240910T070000Z DTEND:20240913T110000Z DESCRIPTION:Educators:\nPavan Videm\, Teresa Müller (RBC)\n\nDate:\n10.09. 2024 – 13.09.2024\n09:00 to 13:00 (CEST)\n\nLocation:\nOnline\n\nContent s:\nThis practical introduction to bioinformatics will explain key concept s of sequencing data analysis as well as provide a broader overview on bio informatics data analysis.\n\nData analysis is a job in high demand. As ea rly as 2012\, a Harvard Business Review article declared data science the “sexiest job of the 21st century”\, and it remains an excellent career choice in “2022 and beyond”. Furthermore\, a study of the healthcare job market during COVID-19 clearly showed a shift towards analytical skill s as a requirement in new job offers.\n\nRNA-seq has revolutionised genomi cs and health care research by being a high-throughput and comparatively l ow-cost method. Along with the development of computational tools for its analysis\, RNA-seq has fundamentally improved our understanding of RNA bio logy.\n\nGalaxy is a global open-source project with the European Galaxy s erver being the largest instance in Europe\, serving more than 70\,000 use rs. The Freiburg Galaxy Team hosts this server out of Freiburg. Through Ga laxy as a gateway\, we offer free access to databases and more than 3\,200 bioinformatics tools which can be used through a graphical user interface . \n\nIn this workshop\, you will learn how to use Galaxy and how to perfo rm a complete RNA-seq data analysis. However\, RNA-seq data is just one of many different kinds of data analysed in bioinformatics. \n\nTo provide a n outlook on what is possible in the field of bioinformatics beyond RNA-se q data analysis\, this course includes additional training materials that will be linked at the end of the course. You are encouraged to review this material on your own during the week following the course. To address yo ur questions about the additional materials and other course content\, we will provide an open question document where you can submit any course rel ated questions during this week.\n\nLearning Goals:\nBy the end of this wo rkshop\, you will be able to:\n\nInteract with the data analysis platform Galaxy\nGet data from external sources into Galaxy\nRun tools on Galaxy an d create a workflow\nPerform and interpret quality control of sequencing d ata\nMap reads on a reference genome and evaluate the mapping quality\nExp lain the principle and specificity of mapping of RNA-seq data to an eukary otic reference genome\nSelect and run a state-of-the-art mapping tool for RNA-seq data\nEstimate the number of reads per gene\nExplain the count nor malisation to perform before sample comparison\nConstruct and run a differ ential gene expression analysis\nAnalyse the DESeq2 output to identify\, a nnotate\, and visualise differentially expressed genes\nUnderstanding diff erent bioinformatic data formats\nPrerequisites:\n\nNo formal training in bioinformatics or genetics is needed. Only basic knowledge of genetics is required\, like: What is DNA\, RNA? or What is a gene?\nKnowledge about se quencing techniques is a plus\nThere is no need for programming or informa tics skills \nPC/Laptop with an up-to-date browser. Chrome\, Safari and Fi refox browsers are all supported (some older browsers\, including Internet Explorer\, may not be)\nRegister a Galaxy EU account before the workshop\ nEuropean Galaxy Server (https://usegalaxy.eu)\nVerify the confirmation em ail!\nIf you have any problem please contact: contact@usegalaxy.eu\nInstal l the genome browser IGV if you would like you would like to inspect your data outside Galaxy\n \n\nKeywords:\nTraining\, RNAseq\, Galaxy \n\nTools: \nGalaxy SUMMARY:A practical introduction to bioinformatics and RNA-seq using Galaxy URL;VALUE=URI:https://www.denbi.de/training-courses-2024/1750-a-practical-i ntroduction-to-bioinformatics-and-rna-seq-using-galaxy END:VEVENT END:VCALENDAR