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DTSTAMP:20260618T114650Z
UID:b4cb69dd-b8d3-40eb-b86e-c56a358cab41
DTSTART:20201123T090000Z
DTEND:20201127T170000Z
DESCRIPTION:Educators:\nDirk Benndorf\, Stephan Fuchs\, Robert Heyer\, Kay 
 Schallert (BiGi/MetaProtServ)\n\nDate:\n23.11.2020 – 27.11.2020\n\nLocat
 ion:\nMagdeburg\, Germany (Universitätsplatz 2\, Building 28.205)\n\nCont
 ents:\nMicrobiome research is rapidly increasing. Meta-Omics data provide 
 insights into microbial community influencing human health and biochemical
  cycle. Metaproteomics focusses on proteins representing the expressed gen
 es and functions. In contrast to metagenomics or metatranscriptomics\, the
  extraction and the analysis of proteins from human or environmental sampl
 es is challenging.\nThe de.NBI course on Applied Metaproteomics trains the
  participants to apply a complete workflow starting from design of experim
 ents via sample preparation to measurement and bioinformatics analysis of 
 high-resolution MS data. Maximum 10 participants are strongly encouraged t
 o analyze their own samples to start their own metaproteomics project.\nTh
 e workshop takes place at chair of bioprocess engineering at Otto-von-Guer
 icke University Magdeburg. The team of microbial communities head by Dr. D
 irk Benndorf is well experienced in metaproteomics. The samples will be me
 asured on newly purchased timsTOF Pro MS. His group devellopes the MetaPro
 teomAnalyzer service facilitating the analysis of of metaproteomic data. T
 he provision of service is supported by the German Network for Bioinformat
 ics Infrastructure - de.NBI.\n\nMonday\n09:00-10:00   Arrival\n10:00-11:00
    Introduction to metaproteomics (lecture: Dirk Benndorf)\n11:00-12:00   
 Design of experiments and sample preparation (lecture: Robert Heyer)\n12:0
 0-13:00   Lunch\n13:00-18:00   Sample preparation I (protein extraction\, 
 protein quantification: Robert Heyer)\nduring the afternoon short presenta
 tions of participant´s about their samples (each participant max. 10 min)
 \n\nTuesday\n08:00-11:00   Sample preparation II (FASP digest\, SDS-PAGE: 
 Robert Heyer)\n11:00-13:00   Introduction to high resolution mass spectrom
 etry (lecture: Dirk Benndorf)\n13:00-14:00   Lunch\n13:00-14:00   Sample p
 reparation II (peptide extraction: Robert Heyer)\n14:00-16:00   Introducti
 on to the MPA 2.0 (lecture: Kay Schallert)\nnon-stop: measurement of parti
 cipant’s samples I\n\nWednesday\nnon-stop: measurement of participant’
 s samples I: Dirk Benndorf\, Patrick Hellwig\n09:00-11:00   MPA 2.0 exerci
 se I (working with a small reference dataset\, db searching\, metaprotein 
 concept: Kay Schallert\, Robert Heyer)\n11:00-13:00   MPA 2.0 exercise II 
 (loading user db\, BLAST search of unknown hits\, quantitative comparison 
 of datasets\, visualization and data export: Kay Schallert\, Robert Heyer)
 \n13:00-14:00   Lunch\n14:00-17:00   Prophane workflow (lecture and traini
 ng: Stefan Fuchs\, external speaker)\n\nThursday\n09:00-13:00   MPA 2.0 ex
 ercise III (analyzing own data: Kay Schallert\, Robert Heyer)\n13:00-14:00
    Lunch\n14:00-17:00   Training at MS (calibration\, setup of LC-MS metho
 ds\, solvent exchange\, trouble shooting\, Patrick Hellwig)\n\nFriday\n08:
 00-10:00   MPA 2.0 exercise IV (visualization of data: Robert Heyer)\n10:0
 0-12:00   Individual discussion of results and preparation of presentation
 s (Dirk Benndorf\, Robert Heyer\, Kay Schallert)\n12:00-13:00   Short pres
 entations of participant‘s results and conclusion\n13:00   Lunch and dep
 arture\n\nLearning goals:\nTo apply a complete workflow starting from desi
 gn of experiments via sample preparation to measurement and bioinformatics
  analysis of high-resolution MS data.\n\nPrerequisites:\nFee: 30 €\n\nKe
 ywords:\nMicrobiome\, MPA\, Metaproteomics\, MetaProteomeAnalyzer\,\n\nToo
 ls:\nMetaproteomeAnalyzer
LOCATION:Magdeburg
SUMMARY:Applied Metaproteomics Workshop 2020 
URL;VALUE=URI:https://www.denbi.de/training/979-applied-metaproteomics-work
 shop-magdeburg-2020
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