BEGIN:VCALENDAR VERSION:2.0 PRODID:icalendar-ruby CALSCALE:GREGORIAN BEGIN:VEVENT DTSTAMP:20260619T140231Z UID:f54aee1a-2bb9-4ff7-8d8e-a7f2c50a2883 DTSTART:20230918T070000Z DTEND:20230922T150000Z DESCRIPTION:Educators:\nDirk Benndorf\, Patrick Hellwig\, Kay Schallert\, R obert Heyer\, Nico Jehmlich\, Claudia Best (BiGi/MetaProtServ)\n\nDate:\n1 8.09.2023 – 22.09.2023\n\nLocation:\nMagdeburg\, Germany (Universitätsp latz 2\, Building 28.217)\n\nContents:\nMicrobiome research is rapidly inc reasing. Meta-Omics data provide insights into microbial community influen cing human health and biochemical cycle. Metaproteomics focusses on protei ns representing the expressed genes and functions. In contrast to metageno mics or metatranscriptomics\, the extraction and the analysis of proteins from human or environmental samples is challenging. \nThe de.NBI course on Applied Metaproteomics trains the participants to apply a complete workfl ow starting from design of experiments via sample preparation to measureme nt and bioinformatics analysis of high-resolution MS data. Maximum 10 part icipants are strongly encouraged to analyze their own samples to start the ir own metaproteomics project. \nThe workshop takes place at chair of biop rocess engineering at Otto-von-Guericke University Magdeburg. The team of microbial communities head by Dr. Dirk Benndorf is well experienced in met aproteomics. The samples will be measured on newly purchased timsTOF Pro M S. His group devellopes the MetaProteomAnalyzer service facilitating the a nalysis of of metaproteomic data. The provision of service is supported by the German Network for Bioinformatics Infrastructure - de.NBI.\n\nMonday\ n09:00-10:00 Arrival\n10:00-11:00 Introduction to metaproteomics (lect ure: Dirk Benndorf)\n11:00-12:00 Design of experiments and sample prepar ation (lecture: Robert Heyer)\n12:00-13:00 Lunch\n13:00-13:30 Safety i nstructions (Claudia Best)\n13:30-18:00 Sample preparation I (protein ex traction\, protein quantification: Patrick Hellweg)\nduring the afternoon short presentations of participant´s about their samples (each participan t max. 10 min)\n\nTuesday\n08:00-11:00 Sample preparation II (FASP diges t\, SDS-PAGE: Claudia Best)\n11:00-13:00 Introduction to high resolution mass spectrometry (lecture: Dirk Benndorf)\n13:00-14:00 Lunch\n13:00-14 :00 Sample preparation II (peptide extraction: Claudia Best)\n14:00-16:0 0 Introduction to the MPA 2.0 (lecture: Kay Schallert)\n18:00-20.00 si ghtseeing in Magdeburg\nnon-stop: measurement of participant’s samples I \n\nWednesday\nnon-stop: measurement of participant’s samples I: Dirk Be nndorf\, Patrick Hellwig\n09:00-11:00 MPA 2.0 exercise I (working with a small reference dataset\, db searching\, metaprotein concept: Kay Schalle rt\, Robert Heyer)\n11:00-13:00 MPA 2.0 exercise II (loading user db\, B LAST search of unknown hits\, quantitative comparison of datasets\, visual ization and data export: Kay Schallert\, Robert Heyer)\n13:00-14:00 Lunc h\n14:00-17:00 Training at MS (calibration\, setup of LC-MS methods\, so lvent exchange\, trouble shooting\, Patrick Hellwig)\n\nThursday\n09:00-13 :00 MPA 2.0 exercise III (analyzing own data: Kay Schallert\, Robert Hey er)\n13:00-14:00 Lunch\n14:00-17:00 Training at MS (calibration\, setu p of LC-MS methods\, solvent exchange\, trouble shooting\, Patrick Hellwig )\n18:30-20:00 dinner (Restaurant “Lago di Garda”) \n\nFriday\n08:00 -10:00 MPA 2.0 exercise IV (visualization of data: Robert Heyer)\n10:00- 12:00 Individual discussion of results and preparation of presentations (Dirk Benndorf\, Robert Heyer\, Kay Schallert)\n12:00-13:00 Short presen tations of participant‘s results and conclusion\n13:00 Lunch and depar ture\n\nLearning goals:\nTo apply a complete workflow starting from design of experiments via sample preparation to measurement and bioinformatics a nalysis of high-resolution MS data.\n\nPrerequisites: \n- Fee: 100 € \n- Οwn Laptop \n- Own samples could be analyzed while workshop (but we coul d also provide samples) \n\nKeywords: \nMicrobiome\, MPA\, Metaproteomics\ , MetaProteomeAnalyzer\, LOCATION:Universitätspl. 2\, 2 Universitätsplatz SUMMARY:Applied Metaproteomics Workshop Magdeburg 2023 URL;VALUE=URI:https://www.denbi.de/training/1602-applied-metaproteomics-wor kshop-magdeburg-2023 END:VEVENT END:VCALENDAR