The course will provide an introduction to Single Cell Genomics. It covers several aspects of single-cell workflows from experimental design to laboratory hands-on including cell sorting and processing for production of quality samples for sequencing, generation of sequencing data and will introduce tools and metrics for assessing the data quality.

Laboratory practicals will involve cell sorting using FACS AriaFusion, including considerations for different cell types, preparation of samples for Smart-seq, library construction, QC and pooling, loading of the 10X instrument.

Delegates will have the opportunity to review sequencing reports to determine data quality, carry out library and pre-processing QC, produce matrices, identify any filtering required and demultiplexing. All theory lectures and hands-on sessions will include best practices and tips as learned first-hand by EI's own faculty.

The course will consist of a mixture of conceptual lectures, methodological lectures and hands-on sessions in both the laboratory and computational analyses, as well as ample time for group discussions.