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DTSTAMP:20260711T114852Z
UID:3635aaf6-30d4-4045-ada5-e74c859129b8
DTSTART:20211005T090000Z
DTEND:20211005T170000Z
DESCRIPTION:Educators:\nJacobo Miranda Ackerman\, Daniel Krause\, Nils Hoff
 mann (de.NBI\, BioInfra.Prot / LIFS)\n\nDate:\nOctober 5th\, 2021\, 9:00 
 – 12:00 (CEST) &amp\; 13:00 – 17:00 (CEST)\n\nDeadline for Workshop Re
 gistration:\nUntil capacity is reached.\n\nLocation:\nOn-Site\, Regensburg
  (if local COVID-19 rules allow\, on-line otherwise)\n\nContents:\nIn the 
 first part of the course\, we will work through an example for targeted LC
 -MS lipidomics with LipidCreator and Skyline. In the second part of the co
 urse\, we will apply PeakStrainer and LipidXplorer for filtering\, identif
 ication and quantification of lipid signals from shotgun lipidomics. The c
 ourse will consist of a short theory and background overview of the employ
 ed programs complemented by the application of the tools to provided data 
 sets. The third part of this course will work through a typical use-case o
 f downstream data processing of shotgun lipidomics data following MS acqui
 sition with LipidXplorer. We will inspect\, check and normalize the data a
 s well as calculate absolute quantities using internal class-specific stan
 dards with lxPostman. In part four\, we will then perform a qualitative co
 mparison of the lipidomes using the LUX Score lipidome homology.\n\nMornin
 g\n09:00 – 09:45 Welcome and Introduction\n10:00 – 10:30 Targeted Lipi
 domics with LipidCreator and Skyline\n10:30 – 12:00 LipidCreator and Sky
 line Exercises\n\nAfternoon\n13:00 – 14:30 Shotgun Lipidomics with Lipid
 Xplorer\n14:30 – 15:30 Quantification of Shotgun Lipidomics data with lx
 Postman\n15:30 – 17:00 Lipidome comparison with LUX Score\n\nLearning go
 als:\nParticipants will be able to understand and explain the shotgun MS a
 nd targeted LC-MS workflows for lipidomics. They will learn the fundamenta
 ls of the software tools used and how to choose parameters for them. They 
 will learn to understand and interpret the results of each step of the pip
 eline.\n\nPrerequisites:\nBasic knowledge of lipidomics\, analytical workf
 lows in lipidomics and basic familiarity with web-based and desktop applic
 ations. Please note that you may need to install software on your computer
  to fully participate in all exercises which may require the proper rights
 . The workshop will be a mix of small lecture segments and hands-on exerci
 ses. The trainers will be available for questions and assistance during th
 e workshop.\n\nKeywords:\nLipidomics\, Shotgun\, Targeted\, LC-MS\n\nTools
 :\nPeakStrainer and LipidXplorer\, LipidCreator\, Skyline\, lxPostman\, LU
 X Score\n\nRegistration:\nPlease select the “LIFS Training session I &am
 p\; II” in the Workshops section of the conference registration. Capacit
 y is limited to 30 participants.\n\n2G (vaccinated or fully recovered) - w
 ith either certificate of full vaccination or certificate of recovery / po
 sitive PCR test not older than six months. Nose+mouth protection - FFP2 ma
 sk or medical mouth nose protection have to be worn inside of buildings.
LOCATION:Regensburg
SUMMARY:LIFS Course - ILS 2021 & LipidomicsForum 2021 
URL;VALUE=URI:https://www.denbi.de/training/1151-lifs-course-ils-2021-lipid
 omicsforum-2021
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