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VERSION:2.0
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CALSCALE:GREGORIAN
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DTSTAMP:20260703T121133Z
UID:ff7f8bdb-4ba9-4947-9029-69c6c70d1f31
DTSTART:20220324T090000Z
DTEND:20220325T170000Z
DESCRIPTION:Single-nucleus RNA sequencing (snRNA-seq) is a complementary te
 chnology to single-cell RNA sequencing (scRNA-seq) which utilizes isolated
  nuclei instead of intact cells to profile gene expression. Both technolog
 ies became powerful tools for investigating cell types\, dynamic states\, 
 and functional processes at the single-cell level\, profoundly revolutioni
 zing transcriptomic studies. While for scRNA-seq the input material is a f
 reshly isolated tissue\, with snRNA-seq isolated nuclei from frozen or pre
 served tissue can be utilized\, enabling processing of archived samples. F
 urthermore\, snRNA-seq can generate  gene expression profile in cells tha
 t are difficult to isolate (e.g. adipocytes\, neurons\, hepatocytes) or wh
 om size exceeds the  technical capabilities of the single-cell technologi
 es.\n\nTo achieve a successful snRNA-seq experiment\, a key prerequisite i
 s preparing a high-quality a single-nucleus suspension. Isolating good qua
 lity nuclei can be a limiting step when working with fragile\, archived ti
 ssues of variable quality. Furthermore\, each nuclei isolation needs to be
  tailored on the tissue type\, state and complexity\, a “do it all” pr
 otocol being hardly possible. With this two days course we intend to make 
 participants familiar with good-practice-guidelines and methods for nuclei
  isolation for single-nucleus sequencing with practical sessions included.
  The course will cover both theoretical and hands-on sessions on sample pr
 eparation\, tissue lysis\, clean-up techniques\, quality control and data 
 analysis.
LOCATION:Leuven - Campus Gasthuisberg\, Herestraat 49
SUMMARY:Nuclei isolation methods for single-nucleus sequencing
URL;VALUE=URI:https://training.vib.be/all-trainings/nuclei-isolation-method
 s-single-nucleus-sequencing
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