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DTSTAMP:20260614T160843Z
UID:ab28275c-3863-4943-a2bd-ab21ea0a9bd5
DTSTART:20170927T071500Z
DTEND:20170929T143000Z
DESCRIPTION:Next-generation sequencing (NGS) of RNA libraries (RNA-Seq) has
  become increasingly common and it largely replaced microarray technology 
 for gene expression profiling. The aim of this course is to get a deeper u
 nderstanding of RNA-Seq experiments\, providing a theoretical introduction
  to the data processing steps\, together with practical sessions illustrat
 ing the use of the most popular data analysis tools. The classroom size is
  limited to 25 participants to optimize the learning and the interaction w
 ith the instructors. Starting from the raw sequenced data coming from diff
 erent phenotypical samples (e.g disease vs healthy control samples)\, gene
 s which are differentially expressed between the two conditions are determ
 ine. Some strategies are illustrated for detecting alternative splicing pr
 oducts\, predicting novel isoforms and gene fusion events. Some methods fo
 r downstream analysis are described to give insight into how biological kn
 owledge can be generated from RNA-Seq experiments. A lecture on single-cel
 l RNA-Seq will provide an idea of how transcriptome data from individual c
 ells is now emerging as a powerful tool\, allowing the study of cell-to-ce
 ll gene expression heterogeneity in the same tissue.
LOCATION:Campus di Fisciano\, Università degli Studi di Salerno\, Via Giov
 anni Paolo II\, n. 132
SUMMARY:Training Course on Best practices for RNA-Seq data analysis
URL;VALUE=URI:https://elixir-iib-training.github.io/website/2017/09/27/RNA-
 seq-Salerno.html
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