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DTSTAMP:20260711T062811Z
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DTSTART:20211007T030000Z
DTEND:20211007T060000Z
DESCRIPTION:It’s now easier than ever to assemble new reference genomes t
 hanks to hybrid genome assembly approaches which enable research on organi
 sms for which reference genomes were not previously available. These appro
 aches combine the strengths of short (Illumina) and long (PacBio or Nanopo
 re) read technologies\, resulting in improved assembly quality.\n\nIn this
  workshop we will learn how to create and assess genome assemblies from Il
 lumina and Nanopore reads using data from a _Bacillus Subtilis_ strain. We
  will demonstrate two hybrid-assembly methods using the tools [Flye](https
 ://github.com/fenderglass/Flye)\, [Pilon](https://github.com/broadinstitut
 e/pilon/wiki)\, and [Unicycler](https://journals.plos.org/ploscompbiol/art
 icle?id=10.1371/journal.pcbi.1005595) to perform assembly and subsequent e
 rror correction. You will learn how to visualise input read sets and the a
 ssemblies produced at each stage and assess the quality of the final assem
 bly.\n\nAll analyses will be performed using [Galaxy Australia](https://us
 egalaxy.org.au/)\, an online platform for biological research that allows 
 people to use computational data analysis tools and workflows without the 
 need for programming experience. \n\n**Date/time:** 2 - 5pm AEDT/ 1 - 4pm 
 AEST/ 1:30 - 4:30pm ACDT/ 11am - 2pm AWST\, Thursday 7 October 2021\n\n**L
 ead Trainer:** Grace Hall\, Melbourne Bioinformatics\n\n**Learning outcome
 s**\n\nBy the end of this workshop you should be able to:\n\n1. Describe h
 ow Nanopore and Illumina reads can be used together to produce a high qual
 ity assembly\n2. Use genome assembly and polishing programs in Galaxy Aust
 ralia\n3. Assess the quality of a genome assembly with and without a refer
 ence genome\n4. Assemble an unknown\, previously undocumented genome to hi
 gh-quality using Nanopore and Illumina reads\n\nThe workshop will NOT prov
 ide an introduction to the basics of Galaxy. If you would like to learn ab
 out this topic there are several tutorials available via the[ Galaxy Train
 ing Network](https://training.galaxyproject.org/training-material/).\n\n**
 Who the workshop is for**\n\nThis workshop is for Australian researchers w
 ho are or will perform hybrid genome assembly as part of their projects.\n
 \nTo get the most out of the workshops you must have experience with the b
 asics of using Galaxy Australia such as setting up a history\, uploading d
 ata and running tools. It is recommended that you complete the tutorial[ G
 alaxy 101 for Everyone](https://training.galaxyproject.org/training-materi
 al/topics/introduction/tutorials/galaxy-intro-101-everyone/tutorial.html).
 \n\nNo programming experience is required.\n\n**How to apply**\n\nThis wor
 kshop is free but participation is subject to application with selection. 
 **_Applications close at 11:59pm AEST Wednesday 29 September 2021._**\n\nY
 ou must be associated with an Australian organisation and provide an appro
 priate organisational email address for your application to be considered.
  \n\nApplications will be reviewed by the organising committee and all app
 licants will be informed of the status of their application (successful\, 
 waiting list\, unsuccessful) by Friday 1 October 2021. Successful applican
 ts will be provided with a Zoom meeting link closer to the date.\n\n**[App
 ly here](https://hybrid-assembly.eventbrite.com.au/)**\n\nThis workshop is
  presented by the[ Australian BioCommons](https://www.biocommons.org.au/) 
 and[ Melbourne Bioinformatics](https://www.melbournebioinformatics.org.au/
 ) with the assistance of a network of facilitators from the national[ Bioi
 nformatics Training Cooperative](https://www.biocommons.org.au/training-co
 operative).\n\nTo hear when registrations open for other events\, please[ 
 subscribe](https://www.biocommons.org.au/subscribe) to the Australian BioC
 ommons’ eNewsletter.
SUMMARY:WORKSHOP: Hybrid de novo genome assembly
URL;VALUE=URI:https://www.biocommons.org.au/events/hybrid-assembly
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