e-learning
Binning of metagenomic sequencing data
Abstract
Metagenomics is the study of genetic material recovered directly from environmental samples, such as soil, water, or gut contents, without the need for isolating or cultivating individual organisms. Metagenomics binning is a process used to classify DNA sequences obtained from metagenomic sequencing into discrete groups, or bins, based on their similarity to each other.
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- What is metagenomic binning refers to?
- Which tools may be used for metagenomic binning?
- How to assess the quality of metagenomic binning?
Learning Objectives
- Describe what is metagenomics binning.
- Describe common challenges in metagenomics binning.
- Perform metagenomic binning using MetaBAT 2 software.
- Evaluation of MAG quality and completeness using CheckM software.
Licence: Creative Commons Attribution 4.0 International
Keywords: Microbiome, binning, metagenomics, microgalaxy
Competency level: •• Intermediate
Target audience: Students
Resource type: e-learning
Version: 9
Status: Active
Prerequisites:
- Assembly of metagenomic sequencing data
- Introduction to Galaxy Analyses
Learning objectives:
- Describe what is metagenomics binning.
- Describe common challenges in metagenomics binning.
- Perform metagenomic binning using MetaBAT 2 software.
- Evaluation of MAG quality and completeness using CheckM software.
Date modified: 2026-04-09
Date published: 2023-12-05
Authors: 
Fotis E. Psomopoulos, Nikos Pechlivanis, Paul Zierep, Vini Salazar
Contributors: Armin Dadras, Bérénice Batut, Deepti Varshney, Helena Rasche, Nikos Pechlivanis, Paul Zierep, Saskia Hiltemann, Teresa Müller, Natalie Whitaker-Allen
Scientific topics: Metagenomics, Microbial ecology, Sequence assembly
Activity log
