Edinburgh genomics introduction to RNA-seq data analysis

Global learning objectives

• Performing RNA-Seq analysis
• Being able to choose an appropriate strategy {Advanced}
• Recognizing the challenges and pitfalls
• Recognizing issues with the data
• Troubleshooting issues with the data {Advanced}
• Recognizing the importance of experimental designs

Content

• 03Qualitycontrolanddata_preprocessing.pdf
• 03Qualitycontrolanddatapreprocessingtutorial.pdf
• 03Qualitycontrolanddata_preprocessing.md
• 04Mappingtoareference_genome.pdf
• 04Mappingtoareferencegenometutorial.pdf
• 04Mappingtoareference_genome.md
• 05Mappingquality_control.pdf
• 05Mappingqualitycontroltutorial.pdf
• 05Mappingquality_control.md

Data

• Original data: SRP019027
• Protocol: unknown (how to get from original data to subset)
• Forward reads
• Reverse reads