e-learning

Designing plasmids encoding predicted pathways by using the BASIC assembly method

Abstract

Synthetic biology is a novel engineering discipline which requires computational tools for the design of metabolic pathways for the production of chemicals such as SynBioCAD portal which is the first Galaxy set of tools for synthetic biology and metabolic engineering.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How to design plasmids encoding predicted pathways using BASIC assembly method ?
  • How to generate scripts enabling the automatic build of plasmids ?

Learning Objectives

  • Annotate the SBML pathway using Selenzy tool.
  • Design plasmids encoding predicted pathways using rpbasicdesign tool.
  • Generate dnabot scripts enabling the automatic build of plasmids.

Licence: Creative Commons Attribution 4.0 International

Keywords: Synthetic Biology

Competency level: • Beginner

Target audience: Students

Resource type: e-learning

Version: 8

Status: Active

Prerequisites:

Introduction to Galaxy Analyses

Learning objectives:

  • Annotate the SBML pathway using Selenzy tool.
  • Design plasmids encoding predicted pathways using rpbasicdesign tool.
  • Generate dnabot scripts enabling the automatic build of plasmids.

Date modified: 2024-06-14

Date published: 2022-05-12

Authors: Guillaume Gricourt, Ioana Popescu, Jean-Loup Faulon, Joan Hérisson, Kenza Bazi-Kabbaj, Thomas Duigou

Contributors: Helena Rasche, Kenza Bazi-Kabbaj, Saskia Hiltemann

Scientific topics: Synthetic biology


Activity log