Workshops and courses

ELIXIR node event Scywalker for processing long-read single cell RNASeq data

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Date: 5 June 2025 @ 09:00 - 17:00

Language of instruction: English

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Single-cell sequencing is typically done using platforms that generate short reads from the 5' or 3' ends of the transcripts. However, this provides no information on isoform variations in the cells, although it is known that isoforms are highly prevalent in complex eukaryotes. In contrast, long-read sequencing (ONT or PacBio) enables the sequencing of full-length transcripts, capturing all isoform variations in the cell. The scywalker tool was designed to analyze long-read single-cell data to generate gene and transcript count matrices that can be further analyzed to compare gene and isoform expression between samples and cell types. 

Keywords: omics

Venue: Ghent - VIB/UGent FSVM II, Technologiepark 75

City: Zwijnaarde

Country: Belgium

Postcode: 9052

Learning objectives:

  • “Discuss the benefits of using long reads for single-cell sequencing”
  • “Provide a complete overview and description of all the results that are generated (for each output file we will explain what info it contains an how it can be used for follow-up analysis)”
  • “Provide a detailed description of each step in the scywalker pipeline”

Organizer: VIB

Event types:

  • Workshops and courses

Instructors: Peter De Rijk

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