Date: 7 October 2026 @ 09:00 - 17:00

Language of instruction: English

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Understanding transcript diversity at the single-cell level is key when you study gene regulation, cell identity, or disease mechanisms. Traditional short-read sequencing limits your ability to capture isoform variation, which is widespread in complex eukaryotes. You will learn how to interpret the results of Scywalker, a tool designed for processing long-read single-cell RNA-seq data (e.g., ONT or PacBio) to enable full-length transcript analysis. You will follow a step-by-step walkthrough of the Scywalker pipeline to generate gene and transcript count matrices for downstream comparisons of gene and isoform expression across samples and cell types. 

Keywords: Artificial Inteligence, omics

Venue: Antwerp - Campus Drie Eiken UAntwerpen, Universiteitsplein 1

City: Antwerpen

Country: Belgium

Postcode: 2610

Learning objectives:

  • Apply Scywalker to generate gene and transcript count matrices from raw long-read single-cell data
  • Assess the suitability of Scywalker for your own single-cell transcriptomics projects.
  • Explain the content and purpose of each output file generated by Scywalker
  • Interpret Scywalker output to identify gene and isoform expression patterns across samples and cell types
  • Recognize the advantages of long-read sequencing technologies (e.g. ONT PacBio) in capturing isoform diversity
  • “Describe each step of the Scywalker pipeline used for processing long-read single-cell RNASeq data"

Organizer: VIB

Event types:

  • Workshops and courses

Instructors: Peter De Rijk


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