hands-on tutorial

Assemble a small genome from reads in your browser

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A 20-minute hands-on tutorial: assemble a small genome de novo from short reads with fermi-lite and compare the contig with the reference using minimap2, all in the browser with nothing to install.

Licence: Creative Commons Attribution 4.0 International

Keywords: PAF, contigs, de novo assembly, fermi-lite, minimap2, workflow

Competency level: • Beginner

Resource type: hands-on tutorial

Learning objectives:

  • Assemble reads into contigs without a reference
  • Compare a contig with a reference genome in PAF format
  • Find differences between an assembly and a reference
  • Read identity and coverage from a PAF line

Authors: IEETA, University of Aveiro

External resources:
BioChef
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