hands-on tutorial

Clean and merge paired-end reads in your browser

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A 15-minute hands-on tutorial: quality-filter paired-end FASTQ files with fastp, merge overlapping pairs and count merged and unmerged reads with seqtk, all in the browser with nothing to install.

Licence: Creative Commons Attribution 4.0 International

Keywords: fastp, paired-end reads, quality control, read merging, seqtk, workflow

Competency level: • Beginner

Resource type: hands-on tutorial

Learning objectives:

  • Filter pairs by quality with fastp
  • Handle the two files of paired-end sequencing together
  • Merge pairs whose reads overlap
  • See how fragment length decides whether a pair can merge

Authors: IEETA, University of Aveiro

External resources:
BioChef
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