hands-on tutorial

Compare two related genomes in your browser

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A 15-minute hands-on tutorial: compare the genomes of phages PhiX174 and S13 with minimap2 in assembly mode, read the PAF output and interpret a rotated circular genome, all in the browser.

Licence: Creative Commons Attribution 4.0 International

Keywords: PAF, genome comparison, minimap2, phage, sequence identity, workflow

Competency level: • Beginner

Resource type: hands-on tutorial

Learning objectives:

  • Align a whole genome against another with minimap2
  • Read identity and differences from PAF output
  • See how the choice of reference changes the coordinates
  • Understand why circular genomes can align in pieces

Authors: IEETA, University of Aveiro

External resources:
BioChef
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