UniProt provides a detailed, high-quality, and freely accessible database of protein sequences and their functional information for the scientific community. More than 50% of these well-curated proteins are enzymes that catalyse a variety of chemical reactions across the kingdoms of life.

This webinar will illustrate the data provided by the UniProt knowledge base (UniProtKB) on enzymes and the ways to access it. Enzyme data can be found in multiple sections of a UniProt protein entry page, which can be used to gain a comprehensive insight into enzyme function, the reactions they are involved in, and how this affects the biological environment around them. We will investigate enzyme annotation through Rhea, a detailed expert-curated database of biochemical reactions that applies the ChEBI ontology to outline reaction participants, their chemical structures, and the chemical changes involved. Furthermore, we will discuss how this information can shape research questions and analyse the enzyme active site, which can influence the design of inhibitors for drug discovery.